Simultaneous determination of triglycerides and cholesterol esters in serum by infrared spectrophotometry
نویسندگان
چکیده
منابع مشابه
Simultaneous Determination of Triglycerides and Cholesterol Esters in Serum by Infrared Spectrophotometry.
With the use of a high-resolution grating infrared spectrophotometer, it is possible to analyze mixtures of cholesterol esters and triglycerides on the basis of the difference in their ester carbonyl absorption frequencies. In the analysis of serum lipids, the preliminary removal of phospholipids is accomplished by a simple batch adsorption on silicic acid. Infrared absorbance measurements are ...
متن کاملSimultaneous determination of triglycerides and cholesterol esters in serum by infrared spectrophotometry
With the use of a high-resolution grating infrared spectrophotometer, it is possible to analyze mixtures of cholesterol esters and triglycerides on the basis of the difference in their ester carbonyl absorption frequencies. In the analysis of serum lipids, the preliminary removal of phospholipids is accomplished by a simple batch adsorption on silicic acid. Infrared absorbance measurements are ...
متن کاملSimultaneous colorimetric determination of cholesterol and triglycerides in serum with a dual-channel autoanalyzer.
A simultaneous, semi-automated method for the determination of cholesterol and triglycerides in serum is presented. The procedure is based upon colorimetric reactions with a 1:10-isopropanol extract of 0.5 ml serum. Both parameters can be analyzed with an output of 30 samples/h in a dual-channel AutoAnalyzer. The flow systems and the preparation of several reagents were improved and simplified ...
متن کاملReagent-free, simultaneous determination of serum cholesterol in HDL and LDL by infrared spectroscopy.
BACKGROUND The purpose of this study was to assess the feasibility of infrared (IR) spectroscopy for the simultaneous quantification of serum LDL-cholesterol (LDL-C) and HDL-cholesterol (HDL-C) concentrations. METHODS Serum samples (n = 90) were obtained. Duplicate aliquots (5 microL) of the serum specimens were dried onto IR-transparent barium fluoride substrates, and transmission IR spectra...
متن کاملNew system for automated extraction and simultaneous determination of serum cholesterol and triglycerides.
We describe an automated system for serum lipid extraction and simultaneous determination of cholesterol and triglycerides, with use of continuous-flow equipment. A sample volume of 100 mul of serum is required, and samples are processed at the rate of 20 per hour, which may be increased with slight loss in precision. Time from sample pickup to recorder readout is about 25 min. The system makes...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1964
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)40244-5